I have the same problem. Windows 10 with creators update. I was using 2.3.1 no problems. Installed 2.5 and it started after installation, but i get the same insert disk message when trying to start it again. I uninstaled all versions of cura and local app data and any additional files i could locate. Downloaded a fresh 2.5, disabled anti virus and firewalls and installed as administrator. It ran from the install complete screen again then when attempting to start again i get the same insert disk error if you repeatedly press continue it will eventually run, but during use i periodically get the insert disk message and have to repeatedly press continue until it clears. If anyone has a solution i would appreciate it.