Have you checked your code? I know people that kept shouting about "my extruder never does anything", but in the end it was just a simple piece of code that kept failing:
G1 F0.0 (aka. do not use stepper motors to move). Just check if there is anything saying 0.0, that could be the cause of your problems.
About the non-sticking issue, what I do (especially with prints like this) is first level the bed, then slowly turn the bed up until you can see that the hotend is actually pushing the layer on the bed, instead of depositing it on top. This way I am sure that the layers are actually sticking to the bed and wont come off while printing.
Try printing this testcube:
It will make 2 spins to get the flow going before actually building the object, make sure that when its doing its first 2 turns that the filament is sticking to the bed. After that, you should get a good test cube. If not, please post a picture of what goes wrong.
Tips to remember:
Layer not sticking to bed: Turn the Z-axis screw left.
Nothing gets deposited, but the extruder screw is turning: Turn the Z-axis screw right, until you see filament getting deposited.
Another problem that I recently found out is that some people experience problems with their filament. What filament are you using?
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Daid 304
Maybe your extruder motor driver is overheating. See:
http://wiki.ultimaker.com/Electronics_b ... or_drivers
Did you calibrate NetFabb?
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