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If the card is inserted after the printer has been turned on, then it may have to be "Initialized".  There should be an option for that in the LCD menu.

You may want to try inserting the card and then turning the printer on.

Another thing to try is to reformat the memory card to FAT32.

Some 3d printers do not read cards over 32mb.

Some machines do not read file names over 16 characters so keep file names on the memory card short.

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