I can't operate the extruder manually

[mashiro 0]

I want to extrude the filament for a certain distance for the calibration of the extruder of the nycubic i3 Mega. I tried the G1 E100 F100 and it failed 

Edited Sunday at 11:53 PM by mashiro

[Slashee_the_Cow 443]

Did you make sure to heat up the hot end first? Marlin will prevent cold extrusion by default.

To heat up the hot end it's M109 S<temperature> so for example M109 S200

[mashiro 0]

M109 S200 succeeded, G1 E100 F100 failed 

[Slashee_the_Cow 443]

5 hours ago, mashiro said:

M109 S200 succeeded, G1 E100 F100 failed 

Are you sending it messages in a way that you can get responses? If you can, post any responses you get from it here.

It's also worth noting that Marlin uses speeds in millimetres per minute so the problem could be the speed. Try G1 E100 F1200 which is 20mm/s. If that doesn't work try F2400.

[mashiro 0]

Sending using the monitor's G-code。 G1 E100 F1200、F2400 failed   

[Slashee_the_Cow 443]

The USB printing feature in Cura is deprecated and no longer updated. You could try running more fully featured software designed for this sort of thing like OctoPrint which has a much more robust terminal.

 

Also, I'm not familiar with your printer, but almost any printer running Marlin should have the ability the control the extruder position using the printer's control panel. You may be best off using that if you can so you don't have to mess with gcode if you can avoid it.