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Threaded rod of z axis is shaking while moving up and down the build plate.

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Its not really a big issue and shouldn't effect printing at all. the 2 guides on the sides are what keeps the bed nice and flat, the lead screw just makes it go up and down. The rod might have a slight bent in it.

You could try to loosen off the stepper motor when the bed is low then tighten it back up. This might help centralize the motor which could lessen the effect.

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