printing at 82% is about right for a very fast print. Illuminarti was able to get it down to 72% with very high pressures (fast extrusion rates). See this article for an explanation - especially look at the graphs (I like graphs) but if you really want to understand it you should probably read the whole article:
http://www.extrudable.me/2013/04/18/exploring-extrusion-variability-and-limits/
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illuminarti 18
Were you pushing filament out through the nozzle when you were testing the extrusion amount?
If so, and you were moving it quickly, then you would have created huge back pressure, leading to filament slipping and under extrusion. Bear in mind that in normal printing, the filament is advancing at 1.5mm/s or even less. (1mm advanced pushes about 6.5mm³ of plastic, so 1.5mm in a second is the getting up towards the 10mm³/s limit). That's equivalent to an F rate of 90 (mm/min).
When you are testing to calibrate E, you need the nozzle hot (so as not to trip the prevent-cold-extrudes) logic. But then use fresh plastic, and insert it so that it's only just going into the Bowden - so that the extruder can advance it without having to actually push it through the nozzle.
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