There should be an option to reverse any and all of the axes.

[T1_L 0]

I have a Prusa i3, and for some reason, it thinks that right is left and vice versa. So when the extruder hits the x endstop, it tries to go the opposite direction, but just keeps going into the endstop. I scoured settings for a motor reversal setting, and it isn't there. I would love it if it was put into the app in the future.

[P3D 46]

Firstly, one word of advice, you probably have much better chances of someone being able to help you with your problem if you post it in a Prusa Forum, not the Ultimaker Forum.

 

Secondly: Are you sure the motor is wired/plugged in correctly? Sounds like a hardware issue to me...

[Smithy 1,145]

I moved your posting to the Cura section, because your posted in Firmware.

 

But Cura has some Prusa profiles and they work out of the box. I did a test with my Prusa and Cura and had no problems.

Are you sure you have selected the correct profile in Cura?

[ahoeben 1,968]

There is nothing in Cura that lets you reverse motor directions, so selecting the proper printer definition is not going to help. You have to fix either the hardware (flip the stepper motor plug on the controller board) or the firmware.

[P3D 46]

See also this thread in the Prusa Forum: https://forum.prusaprinters.org/forum/original-prusa-i3-mk2-s-others-archive/stepper-motor-running-opposite-direction/