No. They multiply together.
So in cura if you set flow to 80%, Cura multiplies all the E values (extruder positions) by 80% such that you get 80% flow.
With the M221 gcode, Marlin (the firmware on the computer) multiplies each of these E values again by the current Marlin flow value. The Marlin flow value of course is set by M221.
If you go into the TUNE menu on Ender (does ender have a tune menu? Probably). and adjust the flow, it will set the Marlin flow value at that moment and forget. If later an M221 value appears - that will override. If later you set it in the TUNE menu again, then at that moment it will override again.
But the flow in Cura is baked right into the E values.
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batesman 1
Got my answer (don't know why I didn't think of this before). I set the flow to 80% in the profile and left the machine settings at 104%. Then I printed a 25 mm cube with .48 wall thickness. The extruded wall thickness was .40 so...There you go. Profile settings override machine settings.
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