The TweakAtZ plugin has to pass at least one layer without change to become active. So the earliest layer you can tweak is layer no. 1, i.e. the second layer (as layer numbering starts with 0). This makes sense as you should put the settings for the first layer into the standard inputs of Cura (UM1) or into the machine (UM2). Never use the TweakAtZ for level no. 0.
IMHO the case that some changes are made at correct level and others are not is highly unlikely (gcode example please).
I don't know but you should play with it a little. You can see the changes easily in the gcode - just open in a text editor (like notepad) and use the editors search feature to search for "50" or "230". Or maybe set the temperature to 231 if there are lots of "230" already in there and speed to "51" if there are lots of "50" already.
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